The Gold Dot NR2 Peptide test is a Magnetic Particle-based enzyme-linked immunosorbent assay (MP-ELISA) intended for the quantitative determination of NR2 peptide fragment of NMDA receptors in plasma. The test is intended to be used in conjunction with clinical evaluation and radiological methods for diagnosis of ischemic stroke and transient ischemic attack (TIA). The Gold Dot NR2 Peptide test correlates with acute TIA and ischemic strokes affecting areas of cerebral cortex. The test allows differentiation between acute cerebral ischemic event and hemorrhagic stroke and stroke mimics.